Chiral technology - enzymatic resolution with bulk enzymes
 

ChiralVision is the specialist in enzymatic preparation of chiral molecules. By using only cheap hydrolytic bulk enzymes, enzyme costs per kilogram of end product can be kept to an absolute minimum. Furthermore, because the enzymes are freely available on the market a steady supply and the lowest possible price is guaranteed. The target for enantiomeric excess is >99%.

Successful approach

By using up to 75 different hydrolytic enzymes in combination with clever substrate derivatisation a very high success rate is achieved. No expensive designer enzyme is needed to get an enantiomeric excess of over 99%. In many cases racemisation of the unwanted isomer is possible. Subsequent recycling leads to a process with high yields.

Field of application

Almost any chiral compound is accessible via enzymatic resolution. It is particularly applicable for chiral:

- alcohols and diols

- amines amides a-amino acids  (also with multiple chiral centers)

- b-amino acids

- amino alcohols

- acids

- esters

 

 

Chiral technology - asymmetric synthesis

 

Alternatively, in case the synthesis of the target structure requires the use of a specialty enzyme for enantioselective synthesis, a large collection of these enzymes are available. Virtually any functionalisation can be introduced enzymatically:
 
Ene reductases:                  asymmetric double bond reduction (23 enzymes)
Ketoreductases:                  asymmetric ketone reduction into chiral alcohols (121 enzymes)
Nitrile hydratases:               synthesis of chiral amides from nitriles (24 enzymes)
Nitrilases:                           synthesis of chiral carboxylic acids from nitriles (40 enzymes)
Oxynitrilases:                      asymmetric synthesis of chiral cyanohydrins (18+ S- and 11 R-enzymes)
ω-Transaminases:               synthesis of chiral amines from ketones, ketoacids (30 enzymes)

microbial pig liver esterase  resolution of racemic esters(6 enzymes)

aldolase (DERA)                 enantioselective C-C bond formation, generating up to two chiral centers (6 enzymes)

cytochrome P450 monooxygenase, hydroxylation of unreactive carbons (5 enzymes)

alcohol oxidase                   enzymatic oxidation of alcohols towards aldehydes with oxygen  (5 enzymes)

nitro reductase                   synthesis of amines from corresponding nitro compounds (12 enzymes)

amidase                             regio- and stereoselective synthesis of chiral carboxylic acids (16 enzymes)

D-amino acid dehydrogenase,   asymmetric synthesis of D-amino acids from keto acids (7 enzymes)

epoxide hydrolase               stereoselective hydrolysis of epoxides towards chiral alcohols (2 enzymes)

Leucine dehydrogenase       acting on CH-NH2 group with NAD+ or NADP+ as acceptor.