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Publications

Scientific publications using ChiralVision products

Overview of publications and research documents in which ChiralVision products were used, ordered from newest to oldest. We hope these publications can act as an inspiration for your enzyme immobilization project. Feel free to contact us at any time.

1. Biobased and Biodegradable Furandicarboxylate Polyesters: Linking Molecular Structure to Enzymatic Hydrolyzability

Date: 21 June 2026  |  Type: Peer-reviewed article

Authors: Sander et al.

Journal / source: Environmental Science & Technology

Chiralvision product(s): HiC / StickAway NZ51032 cutinase

Result / outcome: Linked molecular structure of furandicarboxylate polyesters to enzymatic hydrolyzability; relevant ChiralVision cutinase used for enzymatic biodegradability testing.

2. Substrate Walking for Selective Ene Reduction

Date: 09 May 2026  |  Type: Peer-reviewed article

Authors: Brasselet et al.

Journal / source: ChemCatChem

Chiralvision product(s): Cellulose beads

Result / outcome: Co-immobilized YqjM with glucose dehydrogenase on Ni-NTA cellulose beads; >99% immobilization efficiency, high operational stability, and full conversion on 200 mL scale over 9 days.

3. How to Enhance the Applicability of Metal Ion Affinity-bound Biocatalysts? Comprehensive Overview and Case Study with a Phenylalanine Ammonia Lyase and an Ammonia Lyase from Petroselinum crispum

Date: 24 April 2026  |  Type: Peer-reviewed article

Authors: Laurinyecz et al.

Journal / source: ChemBioChem

Chiralvision product(s): IB-COV-1 beads

Result / outcome: High yields and excellent enantiomeric excesses in ammonia addition; supports were partly reusable/reloadable.

4. Investigation of regioselectivity and thermostability of free and immobilized Pleurotus citrinopileatus lipase

Date: 07 April 2026  |  Type: Peer-reviewed article

Authors: Henrich et al.

Journal / source: Applied Microbiology and Biotechnology

Chiralvision product(s): 17 IB-HIS carriers

Result / outcome: Screened IB-HIS carriers for regioselectivity, thermostability and recycling; IB-HIS-19 was selected as an appropriate immobilization carrier.

5. Lipase-Catalyzed Synthesis of the Antidepressant Moclobemide

Date: 21 November 2025  |  Type: Peer-reviewed article

Authors: Díaz-Fierro, de Prado & Gotor-Fernández

Journal / source: Organic Process Research & Development

Chiralvision product(s): Candida antarctica lipase A (CAL-A)

Result / outcome: Gram-scale synthesis of moclobemide with 86% isolated yield.

6. Enzymatic Hydroesterification of Soybean Oil Deodorizer Distillate: Sustainable Synthesis of Ethyl and Xylose Fatty Acid Esters

Date: 01 November 2025  |  Type: Peer-reviewed article

Authors: Vieira et al.

Journal / source: Fermentation

Chiralvision product(s): IMMTLL-COV-1 and IMMAPF-COV-1

Result / outcome: High xylose fatty acid ester formation: 89.20% with IMMAPF and 80.20% with TLL; products showed suitable emulsifying properties and LCA supported a more sustainable enzymatic route.

7. Candida antarctica Lipase B (CALB)-mediated chemoenzymatic oxidative cleavage of substituted catechols to synthesise muconic acids

Date: 04 October 2025  |  Type: Preprint / SSRN

Authors: Tay et al.

Journal / source: SSRN preprint

Chiralvision product(s): CALB

Result / outcome: Two-pot chemoenzymatic conversion of substituted catechols to muconic acids; yields up to 56% with trace muconolactone by-product.

8. SDS/Carbomer Dual-Stabilized Emulsion: An Efficient System for CALB Immobilized on Acrylic Supports

Date: 01 October 2025  |  Type: Peer-reviewed article

Authors: Siódmiak et al.

Journal / source: Catalysts

Chiralvision product(s): CALB, IB-D152, IB-A161, IB-S60S

Result / outcome: CALB on IB-D152 showed the highest relative activity in the tested emulsion system; dual stabilization significantly enhanced immobilized CALB activity.

9. Emulsion Systems Stabilized with Nonionic Emulsifier and Carbomer: A New Approach for Lipase-Catalyzed Biotransformations

Date: 01 October 2025  |  Type: Peer-reviewed article

Authors: Siódmiak et al.

Journal / source: Catalysts

Chiralvision product(s): CALB and Immobead D152

Result / outcome: Dual emulsion stabilization improved emulsion stability and enhanced lipase activity for scalable high-performance biocatalysis.

10. CALB Immobilized on Octyl-Agarose—An Efficient Pharmaceutical Biocatalyst for Transesterification in Organic Medium

Date: 01 July 2025  |  Type: Peer-reviewed article

Authors: Siódmiak et al.

Journal / source: International Journal of Molecular Sciences

Chiralvision product(s): CALB

Result / outcome: Efficient immobilized-CALB system for pharmaceutical transesterification in organic medium.

11. Carrier immobilization and auto-exposition favoring reuse of silyletherase SilE-OR

Date: 29 May 2025  |  Type: Peer-reviewed article

Authors: Pick et al.

Journal / source: Biotechnology Letters

Chiralvision product(s): IB-S60P, IB-A369, IB-COV-5, IB-COV-7, IB-COV-9

Result / outcome: Carrier immobilization favored reuse of the biocatalytic system with high activity and enantiospecificity in Si-O bond cleavage.

12. Assessing the Industrial Edge of the Lipase-Mediated Oxidation of 2,5-Diformylfuran to 2,5-Furandicarboxylic Acid

Date: 18 April 2025  |  Type: Peer-reviewed article

Authors: Le et al.

Journal / source: Organic Process Research & Development

Chiralvision product(s): IMMCALB-ADS-10

Result / outcome: Lipase-mediated oxidation to FDCA; optimized rotating-bed/reactor-solvent approach reached up to ~27 g/L FDCA.

13. Enantioselective synthesis of (R)-citronellal from geraniol by an immobilised concurrent enzymatic cascade

Date: 03 March 2025  |  Type: Peer-reviewed article

Authors: Tagliabue et al.

Journal / source: Microbial Cell Factories

Chiralvision product(s): Polyacrylic carriers in the screening

Result / outcome: Concurrent cascade achieved 95% conversion to (R)-citronellal with 96.9% ee after 7 h from 10 mM geraniol.

14. Enzymatic Synthesis of Bioactive Structured DHA Phospholipids via Stable Immobilized Biocatalysts

Date: 01 February 2025  |  Type: Peer-reviewed article

Authors: Garcia-Quinto et al.

Journal / source: Processes

Chiralvision product(s): IB-ADS-3

Result / outcome: Use of ChiralVision carrier in synthesis of structured DHA phospholipids; focused on stable immobilized biocatalysts for bioactive lipid synthesis.

15. Designed Reactive Natural Deep Eutectic Solvents for Lipase-Catalyzed Esterification of Phenylacetic Acid

Date: 01 February 2025  |  Type: Peer-reviewed article

Authors: Buzatu et al.

Journal / source: Molecules

Chiralvision product(s): CALA

Result / outcome: Reactive natural deep eutectic solvents improved esterification activity and thermal stability of free and immobilized CaLA.

16. Enzyme-catalyzed polyurethane adhesive degradation

Date: 01 December 2024  |  Type: Peer-reviewed article

Authors: Romano et al.

Journal / source: Reaction Chemistry & Engineering

Chiralvision product(s): HiC cutinase

Result / outcome: Cutinase enabled strong polyurethane adhesive degradation, including up to 87.1% weight loss after 96 h for one PUR formulation.

17. Correlation of Enzymatic Depolymerization Rates with the Structure of Polyesters

Date: 15 October 2024  |  Type: Peer-reviewed article

Authors: Various authors

Journal / source: Macromolecules / polymer degradation study

Chiralvision product(s): Humicola insolens cutinase / StickAway

Result / outcome: Linked polyester structure to enzymatic depolymerization rates; ChiralVision enzyme supported depolymerization testing.

18. Diaminated Cellulose Beads as a Sustainable Support for Industrially Relevant Lipases

Date: 20 May 2024  |  Type: Peer-reviewed article

Authors: Califano et al.

Journal / source: International Journal of Molecular Sciences

Chiralvision product(s): IB-COV-1

Result / outcome: Commercial acrylic beads used as a benchmark while developing sustainable enzyme carriers for industrially relevant lipases.

19. A New Approach in Lipase-Octyl-Agarose Biocatalysis of 2-Arylpropionic Acid Derivatives

Date: 07 May 2024  |  Type: Peer-reviewed article

Authors: Siódmiak et al.

Journal / source: International Journal of Molecular Sciences

Chiralvision product(s): CALB

Result / outcome: Applied CALB/octyl-agarose systems to the biocatalysis of chiral 2-arylpropionic acid derivatives.

20. Different Substrate Selectivity and Product Patterns of Immobilized Thermophilic Lipases from Geobacillus stearothermophilus, Anoxybacillus flavithermus, and Thermomyces lanuginosus for Glyceryl Decanoate Synthesis

Date: 01 February 2024  |  Type: Peer-reviewed article

Authors: Najm, Walsh & Park

Journal / source: ChemEngineering

Chiralvision product(s): IMMTLL-COV-1

Result / outcome: Commercial immobilized TLL was the best match for methyl decanoate and useful for glyceryl decanoate synthesis/product-pattern selection.

21. Upgrading Epoxy Supports for Enzyme Immobilization by Affinity Function Doping - Case Study with Phenylalanine Ammonia-Lyase

Date: 01 January 2024  |  Type: Peer-reviewed article

Authors: Alács et al.

Journal / source: Catalysts

Chiralvision product(s): IB-COV-1

Result / outcome: Immobilized phenylalanine ammonia-lyase applied for production of D- and L-phenylalanine derivatives.

22. Investigating recombinant transaminase stability through immobilization

Date: 01 January 2024  |  Type: Master thesis

Authors: Moschopoulou

Journal / source: Lund University master thesis

Chiralvision product(s): IB-HIS-2 and IB-HIS-22

Result / outcome: Evaluated immobilization strategies for recombinant transaminase stability.

23. Biocatalytic route scouting and enzyme screening toward the synthesis of alpha-benzyl L-glutamate

Date: 01 January 2024  |  Type: Peer-reviewed article

Authors: Quaedflieg et al.

Journal / source: Frontiers in Catalysis

Chiralvision product(s): Immobilized proteases, including IMMALC-COV-2 and immobilized Alcalase, Savinase, Esperase and Protex 6L

Result / outcome: Immobilized Alcalase from ChiralVision gave the best protease-screening hit: 81% yield of N-Boc-L-glutamic acid alpha-benzyl ester with low gamma-isomer formation.

24. Sustainable Phenylalanine-Derived SAILs for Solubilization of Polycyclic Aromatic Hydrocarbons

Date: 19 May 2023  |  Type: Peer-reviewed article

Authors: Kapitanov et al.

Journal / source: Molecules

Chiralvision product(s): Proteases/amidase

Result / outcome: Enzymatic degradation of surfactant ionic liquids; several proteases gave 100% ester hydrolysis and P13 gave high amide hydrolysis.

25. Triggering of Polymer-Degrading Enzymes from Layered Double Hydroxides for Recycling Applications

Date: 03 January 2023  |  Type: Peer-reviewed article

Authors: Romano et al.

Journal / source: Polymers

Chiralvision product(s): HiC / NZ51032 cutinase

Result / outcome: Immobilization/release strategy for polymer-degrading enzymes; cutinase selected because of high polyester-hydrolytic activity.

26. Simple Enzyme Immobilization for Flow Chemistry? An Assessment of Available Strategies and Materials

Date: 01 October 2022  |  Type: Peer-reviewed article

Authors: Wäscher et al.

Journal / source: Molecules

Chiralvision product(s): IB-COV-2

Result / outcome: Assessed immobilization of DERA enzymes for flow chemistry applications.

27. The High 'Lipolytic Jump' of Immobilized Amano A Lipase from Aspergillus niger in Natural Origin Oils

Date: 01 August 2022  |  Type: Peer-reviewed article

Authors: Siódmiak et al.

Journal / source: Catalysts

Chiralvision product(s): IB-COV-1, IB-D152, IB-EC1

Result / outcome: Immobilization caused a strong activity increase ('lipolytic jump') in multiple natural-origin oil systems.

28. Enzymatic Poly(octamethylene suberate) Synthesis by a Two-Step Polymerization Method and Characterization of the Polyester

Date: 01 February 2022  |  Type: Peer-reviewed article

Authors: Pfluck et al.

Journal / source: Processes

Chiralvision product(s): PBL powder, IMMCALB-T1-350 / IMM-PBLI and IMML51-IB-COV-1

Result / outcome: Immobilized commercial enzymes gave high polymerization performance: 60-98% yields and better performance than free enzymes; IMM-PBLI selected as the best candidate for follow-up experiments.

29. Chemoenzymatic Stereoselective Synthesis of trans-Flavan-4-ols via Lipase-Catalyzed Kinetic Resolutions

Date: 01 November 2021  |  Type: Peer-reviewed article

Authors: Soto et al.

Journal / source: Catalysts

Chiralvision product(s): Pseudomonas fluorescens AK lipase

Result / outcome: Very high enantioselectivity (E > 200), 50-99% ee for substrate and >99% ee for product.

30. Strategies for the Immobilization of Eversa Transform 2.0 Lipase and Application for Phospholipid Synthesis

Date: 01 October 2021  |  Type: Peer-reviewed article

Authors: Facin et al.

Journal / source: Catalysts

Chiralvision product(s): IB-ADS-3

Result / outcome: High immobilization performance: 160.8 mg enzyme/g support, 80.4% immobilization yield, 100% recovered activity; 63% yield after 6 cycles in glycerophosphocholine esterification.

31. Evaluation of Designed Immobilized Catalytic Systems: Activity Enhancement of Lipase B from Candida antarctica

Date: 01 August 2020  |  Type: Peer-reviewed article

Authors: Siódmiak et al.

Journal / source: Catalysts

Chiralvision product(s): CALB and Immobeads IB-COV-1, IB-COV-2, IB-D152, IB-C435, IB-A161, IB-A171, IB-A369, IB-EC1, IB-S861, IB-S500, IB-S60S, IB-S60P

Result / outcome: Strong activity improvement: up to 43-fold increase with IB-S861 and 178% hyperactivation on IB-D152; high activity across broad pH and temperature ranges.

32. Candida antarctica Lipase A-Based Enantiorecognition of a Highly Strained 4-Dibenzocyclooctynol (DIBO) and Its Application in the Synthesis of Enantiopure DIBO

Date: 14 February 2020  |  Type: Peer-reviewed article

Authors: Sirén et al.

Journal / source: Molecules

Chiralvision product(s): IMMCALA-COV-1 and other lipases

Result / outcome: Lipase-based O-acylation/enantiorecognition screening of a highly strained DIBO alcohol used for PET tracer synthesis.

33. Efficient Biodiesel Production Catalyzed by Nanobioconjugate of Lipase from Pseudomonas fluorescens

Date: 03 February 2020  |  Type: Peer-reviewed article

Authors: Bartha-Vári et al.

Journal / source: Molecules

Chiralvision product(s): Pseudomonas fluorescens lipase

Result / outcome: Efficient biodiesel production using a lipase nanobioconjugate.

34. Safer bio-based solvents to replace toluene and tetrahydrofuran for the biocatalyzed synthesis of polyesters

Date: 07 March 2019  |  Type: Peer-reviewed article

Authors: Pellis et al.

Journal / source: Green Chemistry

Chiralvision product(s): IMMCALB-COV-1 and cutinase IMML51-T1-350

Result / outcome: Bio-based solvents successfully replaced toluene/THF in enzymatic polyester synthesis.

35. Photochemical Transformation of Poly(butylene adipate-co-terephthalate) and Its Effects on Enzymatic Hydrolyzability

Date: 05 March 2019  |  Type: Peer-reviewed article

Authors: De Hoe et al.

Journal / source: Environmental Science & Technology

Chiralvision product(s): FsC cutinase

Result / outcome: Showed how photochemical aging affects PBAT enzymatic hydrolyzability; photostabilizers mitigated negative impacts.

36. 6-O-Glucose Palmitate Synthesis with Lipase: Investigation of Some Key Parameters

Date: 01 November 2018  |  Type: Peer-reviewed article

Authors: Arcens et al.

Journal / source: Molecular Catalysis

Chiralvision product(s): Supported lipases IMMCALB, IMMCALA, IMMRML, IMMTLL, IMMABC, IMML51

Result / outcome: Successful lipase screening and parameter optimization; full conversion to 6-O-glucose palmitate was achieved under optimized conditions.

37. Biodegradation of synthetic polymers in soils: Tracking carbon into CO2 and microbial biomass

Date: 25 July 2018  |  Type: Peer-reviewed article

Authors: Zumstein et al.

Journal / source: Science Advances

Chiralvision product(s): FsC cutinase

Result / outcome: Demonstrated biodegradation of synthetic polymers in soils by tracking carbon into CO2 and microbial biomass.

38. Performance of Different Immobilized Lipases in the Syntheses of Short- and Long-Chain Carboxylic Acid Esters by Esterification Reactions in Organic Media

Date: 27 March 2018  |  Type: Peer-reviewed article

Authors: de Lima et al.

Journal / source: Molecules

Chiralvision product(s): IMMCALB-COV-1, IMMAPF-COV-1, IMMTLL-COV-1

Result / outcome: High conversions in esterifications: 88.9-98.4% for short-chain esters and up to 96% fructose oleate with IMMCALB-COV-1.

39. Development of a lipase-mediated epoxidation process for monoterpenes in choline chloride-based deep eutectic solvents

Date: 01 June 2017  |  Type: Peer-reviewed article

Authors: Ranganathan et al.

Journal / source: Green Chemistry

Chiralvision product(s): IMMCALB-COV-1-XL

Result / outcome: Developed a lipase-mediated epoxidation process for monoterpenes in deep eutectic solvents.

40. High-Throughput Analysis of Enzymatic Hydrolysis of Biodegradable Polyesters by Monitoring Cohydrolysis of a Polyester-Embedded Fluorogenic Probe

Date: 18 April 2017  |  Type: Peer-reviewed article

Authors: Zumstein et al.

Journal / source: Environmental Science & Technology

Chiralvision product(s): FsC cutinase

Result / outcome: Established high-throughput analysis of enzymatic hydrolysis of biodegradable polyesters using fluorogenic probe cohydrolysis.

41. Removal of Endocrine Disrupting Chemicals in Wastewater by Enzymatic Treatment with Fungal Laccases

Date: 01 April 2017  |  Type: Peer-reviewed article

Authors: Becker et al.

Journal / source: Organic Process Research & Development

Chiralvision product(s): IB-EC-1

Result / outcome: Immobilized laccase achieved strong removal of biological activity, including 82% estrogenic activity removal after 24 h and 99% androgenic activity removal after 6 h.

42. Influence of Hierarchical Interfacial Assembly on Lipase Stability and Performance in Deep Eutectic Solvents

Date: 01 March 2017  |  Type: Peer-reviewed article

Authors: Zhao et al.

Journal / source: Journal of Agricultural and Food Chemistry

Chiralvision product(s): CV-CALBY

Result / outcome: Assessed lipase stability/activity and enantioselectivity in hierarchical interfacial assembly systems.

43. Immobilization of alpha-Amylase from Anoxybacillus sp. SK3-4 on ReliZyme and Immobead Supports

Date: 09 September 2016  |  Type: Peer-reviewed article

Authors: Kahar et al.

Journal / source: Molecules

Chiralvision product(s): IB-COV-1, IB-COV-2

Result / outcome: IB-COV-2 gave 100% activity retention; improved pH stability and reusability, with at least 50% activity after 12 reuse cycles.

44. Tailoring the Spacer Arm for Covalent Immobilization of Candida antarctica Lipase B—Thermodynamic, Kinetic and Molecular Modeling Insights

Date: 01 June 2016  |  Type: Peer-reviewed article

Authors: Abaházi et al.

Journal / source: Molecules

Chiralvision product(s): EP CaLB, IB-COV-1

Result / outcome: Commercial ChiralVision CaLB used as a reference in studies on covalent immobilization and spacer-arm design.

45. Electrospun polylactic acid and polyvinyl alcohol fibers as efficient and stable nanomaterials for immobilization of lipases

Date: 01 January 2016  |  Type: Peer-reviewed article

Authors: Sóti et al.

Journal / source: Bioprocess and Biosystems Engineering

Chiralvision product(s): IB-COV-1 / CALB as reference product

Result / outcome: Effective lipase immobilization and application in kinetic resolution of 1-phenylethanol.

46. Macrocyclic Oligoesters Incorporating a Cyclotetrasiloxane Ring

Date: 13 July 2015  |  Type: Peer-reviewed article

Authors: Ganewatta et al.

Journal / source: Biomacromolecules

Chiralvision product(s): Recombinant CALB

Result / outcome: Enzymatic synthesis of macrocyclic oligoesters incorporating a cyclotetrasiloxane ring.

47. Immobilization and Stabilization of Lipase (CaLB) through Hierarchical Interfacial Assembly

Date: 10 November 2014  |  Type: Peer-reviewed article

Authors: Zhao et al.

Journal / source: Biomacromolecules

Chiralvision product(s): Lipase CV-CALBY

Result / outcome: CALB immobilization with repeated magnetic separation while retaining activity.

48. Studies on lipase selectivity

Date: 01 January 2014  |  Type: Doctoral thesis

Authors: Sundell

Journal / source: University of Turku doctoral thesis

Chiralvision product(s): lipase preparations

Result / outcome: ChiralVision lipase preparations included in selectivity studies.

49. Dieselzymes: development of a stable and methanol tolerant lipase for biodiesel production by directed evolution

Date: 13 May 2013  |  Type: Peer-reviewed article

Authors: Korman et al.

Journal / source: Biotechnology for Biofuels

Chiralvision product(s): Immobead 350 oxirane beads

Result / outcome: Developed stable, methanol-tolerant lipases for biodiesel production; immobilization on ChiralVision carriers supported the process.

50. Hydrophilized Silicone Matrix for the Preparation of Stable Carbonyl Reductase Immobilizates

Date: 01 January 2013  |  Type: Peer-reviewed article

Authors: Scholz et al.

Journal / source: ChemCatChem

Chiralvision product(s): IB-A171

Result / outcome: ChiralVision carrier used in an immobilization strategy for carbonyl reductase; hydrophilized silicone matrix enabled stable immobilizates.

51. Lipase-mediated Epoxidation of the Cyclic Monoterpene Limonene to Limonene Oxide and Limonene Dioxide

Date: 01 October 2012  |  Type: Peer-reviewed article

Authors: Wiemann et al.

Journal / source: Zeitschrift für Naturforschung B

Chiralvision product(s): acrylic carriers / immobilized CALB systems

Result / outcome: Limonene epoxidation was achieved under mild conditions; surface properties of acrylic carriers positively affected performance.

52. Studies on chemoenzymatic synthesis: Lipase-catalyzed acylation in multistep organic synthesis

Date: 01 January 2012  |  Type: Doctoral thesis

Authors: Unknown / thesis author

Journal / source: University of Turku thesis

Chiralvision product(s): CAL-A/CAL-B

Result / outcome: CAL-A/CAL-B used in multistep organic synthesis; examples reported high enantiomeric purity.

53. Intermediates for Enantiopure Drugs

Date: 01 January 2010  |  Type: Doctoral thesis

Authors: Turcu

Journal / source: University of Turku doctoral thesis

Chiralvision product(s): Multiple lipases

Result / outcome: ChiralVision lipases used in screening for enantiopure pharmaceutical intermediates.

Our projects
Recent projects & innovations
At ChiralVision, innovation drives everything we do. Our recent projects highlight our commitment to pushing boundaries and delivering transformative results. From developing cutting-edge technologies to implementing sustainable practices
Jan 2025
ChiralVision and Ecovyst announce collaboration. By combining their strengths cutting-edge technologies that improve the efficiency and sustainability of enzyme-based processes are developed.
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July 2025
Sunresin and ChiralVision Expand Strategic Collaboration to Advance Immobilized Enzyme Technologies
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February 2023
ChiralVision participates in NXTGEN Hightech which is made possible in part by a contribution from the National Growth Fund.
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